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KMID : 1240020160200020122
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International Neurourology Journal
2016 Volume.20 No. 2 p.122 ~ p.130
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Increased Expression of Herpes Virus-Encoded hsv1-miR-H18 and hsv2-miR-H9-5p in Cancer-Containing Prostate Tissue Compared to That in Benign Prostate Hyperplasia Tissue
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Yun Seok-Joong
Jeong Pil-Du
Kang Ho-Won
Shinn Helen-Ki
Kim Ye-Hwan
Yan Chun-Ri
Choi Young-Ki
Kim Dong-ho
Ryu Dong-Hee
Ha Yun-Sok
Kim Tae-Hwan
Kwon Tae-Gyun
Kim Jung-Min
Suh Sang-Heon
Kim Seon-Kyu
Kim Seon-Young
Kim Sang-Tae
Kim Won-Tae
Lee Ok-Jun
Moon Sung-Kwon
Kim Nam-Hyung
Kim Isaac-Yi
Kim Ja-young
Cha Hee-Jae
Choi Yung-Hyun
Cha Eun-Jong
Kim Wun-Jae
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Abstract
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Purpose:
Previously, we reported the presence of virus-encoded microRNAs (miRNAs) in the urine of prostate cancer (CaP) patients. In this study, we investigated the expression of two herpes virus-encoded miRNAs in prostate tissue.
Methods:
A total of 175 tissue samples from noncancerous benign prostatic hyperplasia (BPH), 248 tissue samples from patients with CaP and BPH, and 50 samples from noncancerous surrounding tissues from these same patients were analyzed for the expression of two herpes virus-encoded miRNAs by real-time polymerase chain reaction (PCR) and immunocytochemistry using nanoparticles as molecular beacons.
Results:
Real-time reverse transcription-PCR results revealed significantly higher expression of hsv1-miR-H18 and hsv2-miRH9- 5p in surrounding noncancerous and CaP tissues than that in BPH tissue (each comparison, P<0.001). Of note, these miRNA were expressed equivalently in the CaP tissues and surrounding noncancerous tissues. Moreover, immunocytochemistry clearly demonstrated a significant enrichment of both hsv1-miR-H18 and hsv2-miR-H9 beacon-labeled cells in CaP and surrounding noncancerous tissue compared to that in BPH tissue (each comparison, P<0.05 for hsv1-miR-H18 and hsv2- miR-H9).
Conclusions:
These results suggest that increased expression of hsv1-miR-H18 and hsv2-miR-H95p might be associated with tumorigenesis in the prostate. Further studies will be required to elucidate the role of these miRNAs with respect to CaP and herpes viral infection
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KEYWORD
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MicroRNAs, Prostate Neoplasms, Herpesviridae, Prostate Hyperplasia
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